Abstract: The spatial evolution of industrial structure in 10 counties of Guiyang City in the year of 2000 and 2009 was analyzed by using GIS technology and deviational range method. The results showed that the spatial evolution of industrial structure in Guiyang City exhibited great variations. With regards to the problems existed in the space evolution of industrial structure, four strategies were proposed, including the construction of a network for related industrial space system, strengthening the coordination of industrial space layout, increasing research efforts to encouraging innovation, promoting the rational allocation of labor between industries.