Abstract: 【Objective/Meaning】The gathering and upgrading villages account for the majority of villages in China, which are the focus of rural revitalization, while the land system is the foundation and core of rural institutional system. By focusing on the innovation of land system in the gathering and upgrading villages, the overall vision of the rural land reform was broadened to promote the implementation of the rural revitalization strategy. 【Methods/Procedures】By taking the land reform in S village of Sihong County in Jiangsu Province as the research object, the case study and tracking survey methods were used to analyze. 【Results/Conclusions】It was found that the innovation of land system was the key unlocking point of rural revitalization. Its essence was that the optimization of land system achieved the optimal combination of rural production factors, the reconstruction of public space and the remodeling of village community, thus to promote the rural revitalization. However, there were still unsynchronized and uncertain risks of farmers’development in this mode. In the future, the reform of land system in the gathering and upgrading villages should strengthen the implementation of the overall strategy including the macroscopic and microscopic aspects, and ensure the effective operation of the reform practice by improving the top-level design, so as to actively and steadily promote the rural revitalization.