Abstract: Cultural ecology is the foundation of the inheritance and development of a national culture, and is also the prerequisite for the tourism development with national cultural characteristics.In this study, we firstly analyzed the current situation of Dong village culture and ecological characteristics, and then further studied the problems existed in the tourism development of Dong village in Southeast Guizhou.Then several countermeasures on how to protect cultural ecology of Dong village and promote the development of local tourism resources were promoted, which would make profound contribution to the development of economy and society of Dong village.